This water soluble complex exhibits a strong absorbance at 562 nm that is nearly linear with increasing protein concentrations over a broad working range 20 2000 µg ml. Pierce bca protein assay kit is a two component high precision detergent compatible protein assay for determination of protein concentration. The linear range of these assays for bsa is 125 1 000 µg ml whereas with gamma globulin the linear range is 125 1 500 µg ml.
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